2Cryst-Lab: Support Laboratory for Structural Biology and Chemistry.
Facility
The translation in English would be: "The laboratory is primarily dedicated to the preparation of biological samples before conducting measurements using X-rays, such as crystal diffraction (MX) or low/high angle scattering (SAXS, WAXS), using the equipment at our institute and/or synchrotron centers. Preparation involves purifying the samples and, if necessary, subsequently crystallizing them. The most important instrumentations are: - NGC Chromatography System BIORAD / NGC BIORAD Chromatography System. - NIKON MULTIZOOM AZ100 Stereo Microscope and NIKON DS-Fi1 Digital Camera. - Nanodrop UV-Visible Spectrophotometer - Agilent Cary Eclipse fluorescence spectrophotometer
Bio-macromolecule purification, crystallization and characterization by structural biology X-ray techniques
Academic collaboration, Facility, Skill, Software
Our expertise lies in purification and advanced characterization by X-ray-based techniques of bio-macromolecules to perform cutting-edge structural biology investigations on proteins and their complexes. Our proven experience enables us to handle challenging systems such as membrane proteins and protein-ligand complexes. Regarding protein-ligand adducts for crystallographic studies, in addition to standard soaking experiments using single ligands, we also perform fragment screening for rational drug design. We offer the opportunity to perform structural biology studies by X-ray diffraction for high-resolution structure determination and provide established proficiency to obtain crystal structure information about protein-ligand interactions. Moreover, we are experts in Bio Small-Angle X-ray...
Biocrystal Facility
Academic collaboration, Facility, Industrial collaboration, Skill
The Biocrystal facility, a large multidisciplinary laboratory established at the Institute of Molecular Biology and Pathology (IBPM) of CNR, in collaboration with the Biochemistry Department of Sapienza University aims at supporting the Italian scientists and the pharmaceutical companies in the research to find new drug and vaccine against the endemic and epidemic diseases through structure-based drug design. The Biocrystal facility is part of the ITACA.SB INFRASTRUCTURE financed by the Mission 4 / Education and Research Component 2: from research to business, Investment 3.1: Fund for the realisation of an integrated system of research and innovation infrastructures. The main Biocrystal Facility...
Biomolecular NMR: structural and interaction studies of biological macromolecules and drug discovery applications
Academic collaboration, Facility, Research field/topic, Skill
-Instrumentation Our team is provided with a cutting-edge Bruker Avance 500 MHz NMR spectrometer equipped with a nitrogen-cooled cryogenic probe. The advanced technology of this NMR instrument allows us to acquire mono and multi-dimensional NMR spectra of small molecules and macromolecules at low concentrations, due to its high sensitivity. The NMR spectrometer is optimized to study the solution structure and dynamics of proteins and nucleic acids and their interactions with peptides, and small compounds for drug-discovery applications. We can analyze samples under different buffer and temperature conditions and even in the cellular environment. The main components of the NMR spectrometer...
BioSAXS
Facility
BioSAXS will allow to conduct preliminary studies, select the best samples to be analyzed (possibly) in synchrotron light laboratories. Furthermore, if equipped with Size Exclusion Cromathograhy (SEC-BioSAXS) and Ultra Small Angle Scattering (USAXS), it will be possible to study macromolecules, selected by size, up to 1 micron. Finally, a reactivity chamber will allow to manage samples in an inert or controlled atmosphere, avoiding exposure of the substances to oxygen during handling. XENOCS XEUSS3.0 system, HR model, equipped with a motorized dual source: Ga Excillum liquid jet source and Cu micro-source. Detector: Eiger 2R (1M), moving in vacuum from 42.5 mm...
Cellular and Molecular Drug Profiling in Disease Models, ICB facility
Facility, Research field/topic, Skill
Cellular and Molecular Drug Profiling in Disease Models: Cell-based platforms to study drug effects in cancer and neuroinflammation models. Techniques include cell proliferation and survival assays, autophagy analysis, invasion and migration studies, and EMT-stemness profiling. Expertise includes analysis of inflammatory pathways through cytokine/chemokine assessment, immune cell polarization, and FACS-based cell surface marker characterization.
Computational Biology, Computational Enzymology, Drug Discovery, Molecular recognition, Protein-Protein-peptide interaction, Peptide design and synthesis, High Performance Computing, Surface Plasmon Resonance, ELISA, Antibody characterization
Academic collaboration, Facility, Research field/topic, Skill, Software
The Structural Biology Group at CNR-ICCOM focuses on exploring the relationship between the structure, dynamics, and function of biomolecules using a combination of computational biology tools, synthetic chemistry, and biomolecular recognition techniques. Computational Laboratory The group employs advanced methods such as molecular dynamics simulations, docking, drug discovery, and bioinformatics (from quantum to empirical force-fields) to investigate and design biological systems, ranging from small molecules and peptides to proteins and nucleic acids. Key research areas include: 1. Molecular Effects of Sequence-Structure Perturbations: Investigating the impact of binding events and mutations on the activity and interactions of proteins involved in critical biological...
Computational Chemistry and Cheminformatics, StOrMoDB platform, EHBAS platform
Facility, Research field/topic, Skill, Software
The main computational chemistry skills in the ICB Computational Chemistry and Cheminformatics group (ICB-CCC) cover the fields of homology modelling, molecular docking and molecular dynamics simulations of biomolecular systems and quantum chemistry calculations on isolated molecules or their complexes with representative protein fragments. These skills are mainly aimed at studying the driving forces at molecular level of ligand-protein interactions, the molecular bases of receptor selectivity, and the conformational transitions associated to the modulation of receptor activities. As for the main application areas of these techniques and approaches, ICB-CCC devotes a special attention to the characterization of nuclear receptors, GPCRs and...
Computational Chemistry, HPC Clusters, simulation software
Academic collaboration, Facility, Research field/topic, Skill, Software
Computational chemistry and molecular modeling. Both quantum chemistry calculations and classical reactive/non-reactive molecular dynamics simulation methods are employed. These are creatively used to investigate the conformational and physicochemical properties, dynamics, and reactivity of oligopeptides, proteins, enzymes, DNA/RNA/PNA, lipids, micelles, membranes, cellulose-based materials, polymers, small molecules, etc., in the gas phase, in solution (explicit molecules or a continuum medium), and contact with various types of inorganic interfaces (hybrid complexes). The structure and dynamics of metal and metal oxide nanoparticles (various morphologies and sizes) functionalized with different types of molecules are simulated with appropriately parametrized reactive force fields (ReaxFF - missing parameters...
Computational Modelling, HPC Linux cluster, Modelling software, computational biophysics, academic collaborations, industrial collaborations
Academic collaboration, Facility, Industrial collaboration, Research field/topic, Skill
Computational Modelling: The main research directions developed in the lab are in silico drug discovery and modelling of basic biomolecular mechanisms on protein targets. Prediction of structure-function relationships is carried out by using all-atom computational modelling and Molecular Dynamics and by developing specific analysis methods based on statistical mechanics and AI. Computational drug discovery projects are enhanced by machine learning and strongly rely on in silico virtual screening and molecular docking. Starting from a protein target of interest, this approach leads to designing candidate hits as well as predicting their biological activity and pharmacokinetic parameters. Within SCITEC, our work relies...
Cryo-electron microscopy , IOM cryo-EM facility (CMF), Electron Microscope
Academic collaboration, Facility, Industrial collaboration, Skill
Cryo-Electron Microscopy (Cryo-EM) is an advanced imaging technique used to determine the three-dimensional (3D) structure of biological molecules and complexes at near-atomic resolution. In this method, the sample is rapidly frozen at temperatures below -150 °C, encapsulating it in vitreous ice. The specimen is then imaged from various angles using an electron microscope, where a beam of electrons passes through the sample, producing a series of two-dimensional (2D) projections. Advanced computational algorithms are employed to reconstruct a 3D model of the sample from these projections. The CMF is equipped with the necessary instruments to conduct cryo-electron microscopy studies, including single-particle...
CUBE Computational Unit for Biological Exploration
Academic collaboration, Facility, Industrial collaboration, Research field/topic, Skill, Software
Background and skills: Recent years have seen ground-breaking achievements of deep-learning methods applied to the 3D structure predictions of biomacromolecules, with the phenomenal success of AlphaFold software in obtaining protein structures at an unprecedented level of accuracy. The availability of huge data on predicted protein structures is revolutionizing the field of experimental structural biology (based on classical X-ray, NMR and Cry-EM methods) making the frontier between experimental and computational approaches more and more blurred. In this context, it is increasingly important to integrate the outcomes from the different techniques in order to favour the mutual exchange, validation, enhancement of experimental...
Design, Synthesis and Characterization of Natural-Like Phenols and Hydroxylated Biphenyls with Potential Biological Activity
Facility, Research field/topic, Skill
The research activity aims to optimize the use of industrially accessible natural resources on a large scale to enhance the value of their compounds in the service of sustainable development and technological innovation. These actions require expertise and basic research, often challenging to develop within a company or industry for economic and structural reasons. A computational protein-ligand study on specific receptors is used to predict the structure of the target molecule, which guides the synthesis design. The activity focuses on chemical synthesis oriented toward the molecular diversity of small collections of phenols and hydroxylated biphenyls with natural reminiscence to identify...
DEVELOPMENT ON MOLECULAR BASIS OF SELECTIVE BIOACTIVE MOLECULES FOR THE DIAGNOSIS AND TREATMENT OF DISEASES WITH HIGH SOCIAL IMPACT
Facility, Research field/topic, Skill
The research activity is part of the development of new molecules for diagnostic and therapeutic applications with molecular approaches based on the study of the relationships between structure and biological activity aimed at the design and/or identification of selective molecules for the diagnosis and treatment of diseases with high social impact with particular reference to diseases neurodegenerative and oncological diseases with a higher incidence (Alzheimer's and Parkinson's disease, prostate cancer, colon cancer), as well as inflammatory and bacterial infectious diseases. In particular, the topics covered are: a) study of protein/protein and protein/oligonucleotide complexes; b) synthetic systems recognizable by biological targets;...
Drug Design and Development
Academic collaboration, Facility, Skill
Synthesis and conformational analysis of bioactive peptides CD (Jaco J-1500 - DiSC UNIPD); NMR (Bruker Avance-NEO 600 MHz, Bruker 400 Avance III HD - DiSC UNIPD) Peptide-ligand and protein-ligand interaction studies CD (Jaco J-1500 - DiSC UNIPD); ITC (Malvern PEAK-ITC microcalorimeter - DiSC UNIPD) SRCD (synchrotron radiation circular dichroism) e MIRIAM (Multimode InfraRed Imaging And Microspectroscopy) -Diamond Light Source Ltd, Harwell Science & Innovation Campus, Didcot, Oxfordshire (UK) Academic collaboration: Giuliano Siligardi, Rohanah Husain (SRCD) e Gianfelice Cinque (MIRIAM)
Glycomics, Bottom-up proteomics, Structural analysis of Glycoconjugates. Gglycoproteomics and MS laboratories
Academic collaboration, Facility, Industrial collaboration, Research field/topic, Skill
Glycomics: sample preparation from biological fluids, cells and tissues, permethylation analysis, Rapifluor derivatization, HILIC separation, MS detection, MSMS analysis in healthy and pathological conditions (CDGs, neurodegenerations, inflammatory diseases) Proteomics: enzymatic digestions, peptide separation (multi-dimensional), MALDI LC-MS/MSMS, ESI LC-MS/MSMS. Glycoconjugate characterization: LipoPolysaccharides, Lipid-A, core OS, LPS characterization by MALDI MS. Immunopurification of targeted glycobiomarkers. Instrumentation: -MALDI 4800 AB Sciex -MALDI UltraflExtreme Bruker -Exactive Orbitrap Thermo Scientific -Fortis TSQ Thermo Scientific -Exploris 120 Orbitrap Thermo Scientific
GPCR Drug Discovery and Pharmacological Profiling Platform, ICB facility, b-counter, multi-detection plate reader
Facility, Research field/topic, Skill
GPCR Drug Discovery and Pharmacological Profiling Platform: Screening of compound libraries to evaluate binding affinity and functional responses on GPCR targets. Expertise includes pharmacological profiling, mechanism of action studies, and preclinical evaluation of drug candidates across in vitro, in vivo, and ex vivo models, targeting therapeutic applications in cancer and neuroinflammation.
HERMES (High-performance Environment for Research in MolEcular and Structural Biology)
Facility
HERMES (High-performance Environment for Research in MolEcular and Structural Biology), is a high-performance computing cluster designed to provide support to the scientific community for CryoEM, BioSAXS, X-Ray data storage and processing, as well as to enhance theoretical and computational research in Structural Biology and Drug Discovery. Specifically, the system consists of 5 CPU compute nodes and 4 CPU/GPU compute nodes, each with 4 NVIDIA H100 GPUs capable of delivering high computational power for applications such as image processing, artificial intelligence model training, and biological big data processing. Additionally, the system features a Scale-Out NAS storage system capable of supporting the...
In silico Drug Discovery. Instrumentation: Server ; Software: Schrodinger suite and AMBER
Academic collaboration, Facility, Skill, Software
In silico Drug Discovery: Chimico computazionale con una solida formazione in sintesi organica e un’approfondita esperienza nel supporto a programmi di ottimizzazione per la scoperta di farmaci. Competenza nello sviluppo e nell’applicazione di tecnologie di screening virtuale, inclusi modelli farmacoforici basati sulla struttura e sul ligando, nonché calcoli di docking. Esperto in 3D QSAR, modellistica per omologia, ADME-Tox e nell’implementazione di cascate di screening virtuale. Particolare interesse nel riposizionamento di farmaci utilizzando approcci in silico e nell’impiego della genetica per identificare target farmacologici innovativi. La struttura informatica dispone di una capacità di archiviazione e di prestazioni computazionali elevate, adatte a...
In silico structural biology to solve biological mechanisms and guide drug discovery
Academic collaboration, Facility, Patent, Research field/topic, Skill, Software
The Laboratory of Molecular Modeling and Virtual Screening (MMVS_Lab) at SCITEC-Rome integrates diverse computational expertise to investigate biological structures and processes: -Computational Chemistry: molecular dynamics simulations of biological molecules; quantum mechanical methods to analyze molecular properties; molecular docking to predict ligand-protein and protein-protein binding interactions. -Molecular Modeling: structural homology; fold recognition; conformational analysis. -Computer Science and Data Science: use of supercomputers/high-performance computing (HPC) facilities at European computing centers for complex simulations; application of machine learning and artificial intelligence (AI) to predict molecular structures and behaviors. -Bioinformatics: Sequence analysis for the prediction of functional domains and structural features of proteins. -Drug...
Integrative approaches of structural biology by multi-scale imaging applied to single protein for “in vitro” and “in vivo” pre-clinical screening of bioactive compounds. NeuroChemistry and Cell Signaling Lab (CristinoLab)/ Cryostat; Utramicrotome; INCUCYTE SYSTEM; LIVE CELL MICROSCOPY” WORKSTATION; ZOOM/AIVIA/MetaMorph Software.
Academic collaboration, Facility, Industrial collaboration, Research field/topic, Skill
How do molecular machines work, interact, and harmonize their activities to create a fully functional cell? How are these processes regulated in physiological conditions or deregulated in pathological ones like those in human disease? The Structural Biology approach of the Cristino Lab aims to answer these questions by gaining precise knowledge of the spatial identity and distribution of macromolecules and macromolecular complexes within cellular compartments (lysosomes, mitochondria, rough endoplasmic reticulum, Golgi apparatus, membranes, and nucleus), tissues, and anatomically well-defined structures (brain, synapses, choroid plexus, gut barrier, blood-brain barrier, tight junctions, exosomes, nanovesicles, neurovascular unit, neuromuscular junction, retina). The Cristino Lab's...
ITACA.SB Potentiating the Italian Capacity for Structural Biology Services in Instruct-ERIC – RU CNR-IC Caserta
Facility
ITACA.SB project is dedicated to enhancing the structural biology (SB) services offered by the Italian center of Instruct-ERIC, aiming to achieve three main objectives that focus on improving the quality and quantity of the Italian node CERM/CIRMMP’s exploitation by both Italian and European users. This initiative seeks to elevate the level of research in Life Sciences within Italy, enhance international visibility and competitiveness for EC funding, and address specific bottlenecks faced by Italian structural biologists in accessing Instruct-ERIC services. In particular, in the RU in Caserta it was employed the following facilities for Cryo-EM screening and Protein expression: - Glacios:...
Laboratorio di Biologia Molecolare e Strutturale (BMS)
Academic collaboration, Facility, Skill, Software
SKILLS AND COLLABORATIONS The team (see above) uses a wide range of methodologies to unravel complex biology problems, including molecular biology, x-ray crystallography, biophysics, and computational work (molecular modelling and dynamics, docking). The Team actively cooperates with microbiologists, immunologists and cell biologists (e.g. Imperial college London, UCD Dublin, Munich University, Harvard University, Karlsruhe Institute of Technology) and is involved in PNRR projects PE13 INF-ACT, CN1 and CN3. INSTRUMENTATION The molecular biology Labs of this team (Labs 8 and 9) are located in Via Pietro Castellino 111, Naples, and are equipped with (non-exhaustive list) T 100 Thermal Cycler (BIO-RAD), Fume cupboard...
Light scattering, spectroscopy, microscopy, calorimetry, rheology, chromatography, cell culture devices, supercomputing and data-storage resources
Facility, Software
Spectroscopy: JASCO J-815 Circular Dichroism; SHIMADZU UV-VIS Spectrophotometer; JASCO FP6500 Spectro fluorimeter; THERMO Ascent FL Fluoroscan HAMAMATSU C9413 FCS Unit; Spectrofluorimeter CARY Agilent Eclypse, equipped with thermostated control, microplane reader and polarization module Static and Dynamic Light scattering: BROOKHAVEN SM200 Spectrometers BROOKHAVEN BI9000 Correlators BROOKHAVEN BI-DNDC Differential refractometer Homemade CCD Low angle scattering system Calorimetry: TA INSTRUMENTS Nano DSC TA INSTRUMENTS Nano ITC HART 707 Microcalorimeter Microscopy: NIKON Eclipse Ti fluorescence Microscope; JPK AFM NanoWizard 3, coupled with NIKON Eclipse Ti Optical Microscope and Temperature controlled Petri Dish Heater; NIKON Eclipse 80i fluorescence Microscope Rheology facilities: TA INSTRUMENTS AR1000 Rheometer...
Macromolecular Structural Determination of the IBISBA Research Infrastructure
Facility, Skill
I run the Macromolecular Structural Determination of the IBISBA Research Infrastructure @ IBBA Milano
MaSCoT (Molecular, Structural, and Computational Biology – Advanced Research Team)
Facility, Skill
MaSCoT is a multidisciplinary research team with in-depth expertise in cutting-edge techniques for biochemical, structural, and computational studies on macromolecules of biomedical and biotechnological interest. The team benefits from a robust network of national and international collaborations, which includes University of Florence, University of Montpellier, Tampere University, the Latvian Institute of Organic Synthesis, the Slovak Academy of Sciences, and others. MaSCoT includes four closely integrated laboratories: • The Molecular Biology Lab, which uses the most advanced instruments for the design, expression, and biochemical and biophysical characterization of proteins. • The Structural Biology Lab, which is at the forefront of studying...
Mitochondrial Bioenergetics and Oxidative Stress Analysis, ICB facility, high-resolution respirometer (Oroboros)
Facility, Research field/topic, Skill
Mitochondrial Bioenergetics and Oxidative Stress Analysis: Mitochondrial function study using high-resolution respirometry (Oroboros) for precision OXPHOS analysis. Expertise includes investigating oxidative stress via mtROS assays, bioenergetic pathway analysis, and studying metabolic shifts in intact cells, isolated mitochondria, and freshly isolated tissues. Capabilities extend to linking mitochondrial function with cellular metabolism and survival in disease contexts.
MolBD3: Molecular Bases of Diseases and Drug Design
Academic collaboration, Facility, Research field/topic, Skill
Skills in Structural Biology and Protein Biochemistry/Biophysics Experimental Techniques Protein Expression and Purification Production of recombinant proteins in bacterial, insect, yeast, and mammalian systems. Expression of isotopically labeled proteins (¹H, ¹⁵N, ¹⁵N/¹³C). X-ray Crystallography Protein crystallization. Data collection and processing. Structure determination and refinement. Cryo-Electron Microscopy (Cryo-EM) Single-particle reconstruction. Atomic model building. Small Angle X-ray Scattering (SAXS) Solution-based structural analysis. Data modeling and shape reconstruction. Bioinformatics and Computational Tools Protein Structure Modeling Homology modeling. De novo structure prediction. Docking Studies Protein-ligand docking. Protein-protein and protein-DNA/small molecule interaction modeling. Molecular Dynamics Simulations Simulation setup and execution. Analysis of trajectories. Structure Visualization...
Molecular Biology, Structural Biology, Protein-binding studies. Facility: XRD1 beamline, Cryo-EM (late 2025), Binding studies platform. Instruments: FPLCs, shakers, centrifuges, Spark (Tecan), GCI Wave-delta (Malvern), Helix+ (Bruker), ITCs (Malvern), UPLC-MS (mid 2025)
Academic collaboration, Facility, Research field/topic, Skill
Skills - Molecular Biology. The IC-Ts laboratory is deeply involved in structural biology, in this respect the laboratory has a long and important experience in molecular biology, proteins expression and purification. Structural Biology: Among the techniques used in Structural Biology at IC-Ts' laboratory, we have been using X-ray Crystallography, SAXS and NMR. Furthermore, we have recently upgraded the laboratory devoted to Protein-ligand interaction studies. Facility: IC-Ts is strongly involved in synchrotron radiation, we share at 50% XRD1 diffraction beamline with Elettra – Sincrotrone Trieste and we have a facilitated access to the XRD2 beamline. We are part of the PNRR...
Phosphorene Nanoparticles with Photoactive Features for Potential Biomedical Applications
Academic collaboration, Facility, Skill
The PMC_lab at ICCOM, led by researchers Serena Coiai, Francesca Cicogna, and Elisa Passaglia, has extensive experience in the synthesis and characterization of functional nanoparticles designed for advanced polymer-based materials. In recent years, the team has developed expertise in preparing phosphorene nanoparticles through liquid-phase exfoliation processes. These nanoparticles are stabilized using decoration effects and are embedded within biocompatible and biodegradable polymer phases or micelles. The nanoparticles have been characterized to evaluate their potential in generating singlet oxygen upon irradiation, assessing their applicability in photodynamic therapies. Preliminary results, collected in collaboration with the Nanoscience Institute of CNR, have been promising, especially...
Production and characterization of soluble and membrane proteins involved in riboflavin homeostasis and related diseases
Academic collaboration, Facility, Research field/topic, Skill, Software
The laboratory of DBBA of the University of Bari (Convenzione operativa tra IBIOM e Università di Bari, prot n. 0174808 del 24/05/2024) is equipped with a Gilson HPLC system with a FP-2020 Plus Jasco fluorescence detector and Unipoint system software; a spectrophotometer; a FP-3800 spectrofluorometer; and laboratory equipment for molecular biology. At DBBA modern laboratory rooms are organised as multidisciplinary technological core consisting of different platforms: 1) proteomics 2) genomics 3) cellular engineering and biology 4) sub-cellular biochemistry and biophysics 5) Imaging facilities. DBBA also has an animal house and rooms for culturing mammalian cells and bacteria, yeasts and invertebrates...
Protein interactions and dynamics by solution NMR – Istrumentation: NMR spectrometers for solution studies – Advanced NMR techniques for mechanistic investigation of disease related protein systems
Academic collaboration, Facility, Research field/topic, Skill
*SKILLS: - NMR based drug design and optimization - NMR detection for protein-protein and protein/ligand interactions - Time-resolved NMR to follow misfolding and aggregation phenomena - NMR Diffusion methods for interaction and aggregation phenomena characterization - Data driven docking approaches *FACILITY INSTRUMENTATION: SCITEC-CNR has a well-established NMR Facility with 3 solution-state NMR spectrometers operating at 600 MHz, 500 MHz, 400 MHz. 600 MHz Avance Neo Bruker is equipped with triple resonance (1H, 15N, 13C) Prodigy CryoProbe ensuring the sensitivity gain necessary to fully characterize proteins interactions and dynamics employing low protein concentrations 500 MHz spectrometer is equipped with a triple...
Proteomic and Genomic analysis, In silico protein analysis, MALDI-TOF mass spectrometry
Facility, Skill, Software
Expertise in the field of protein study by top-down and bottom-up proteomic approach using LC-MALDI-TOF mass spectrometry. Expertise in human genetic analysis mainly in the field of neurological diseases by DNA sequencing (Sanger) and qPCR. In silico protein 3D structure analysis (using Chimera X software) and Molecular Dinamic Analysis (using Gromacs software) in order to study the functional effect of our sample with human mutated proteins. Collection of viable cellular samples from patients carrying genetic mutations (not certified as a biobank).
Recombinant protein production and purification – Biophysical characterization of proteins – Structural characterization of proteins
Facility, Skill
Recombinant protein production and purification: chromatographic ÄKTA systems. Biophysical characterization of proteins: ITC (Malvern PEAK-ITC microcalorimeter), DLS (Malvern Zetasizer Nano), SAXS (European Synchrotron Radiation Facility - ESRF). Structural characterization of proteins: NMR (Bruker Avance-NEO 600 MHz), crystallography (European Synchrotron Radiation Facility - ESRF), Cryo-EM (Glacios cryo-TEM at DSB-UNIPD)
Single Particle Anaysis, ElectronTomography, Cryo Electron Microscopy
Academic collaboration, Facility, Patent, Research field/topic, Skill, Software
The Cryo Electron Microscopy (Cryo-EM) laboratory at ISASI Naples (EYELAB) offers advanced facilities and expertise for identifying, visualizing, and characterizing biological macromolecules in their native states. It supports a comprehensive approach to studying the structure and function of proteins, nucleic acids, and other macromolecules, as well as their interactions within cells and tissues. The laboratory offers high-resolution structural analysis through Single Particle Analysis (SPA) and Electron Tomography (ET). SPA reveals the 3D structures of proteins, enzymes, and complexes in their hydrated states, providing unprecedented detail on their architecture and function. ET enables 3D visualization of cellular structures, and tissues, shedding...
Spontaneous Raman Microspectroscopy, Scanning Probe Microscopy, Machine Learning and Deep Learning
Academic collaboration, Facility, Industrial collaboration, Skill
My group is focused on the application of Raman spectroscopy to cancer tissues for a complete grading of variuos type of cancers. For such topics we emply machine learing and deep learing techniques for managing large datasets. In addition, we use Raman spectroscopy for a characterization of biological samples, such as cells, etc. Another activity of our group is the synthesis of nanocomposites for antiviral application, making use of electrospinning techniques and drug delivery, nanoparticles etc., making use of microfluidic device. In turn, we are active on quantum biology.
Structural and Dynamic Insights into Oligopeptides through NMR and EPR
Academic collaboration, Facility, Research field/topic, Skill
My recent research focuses on the coordination of copper in oligomers of amyloid-β peptides and in peptides involved in angiogenesis, employing magnetic resonance techniques such as EPR, solution NMR, and fast field cycling NMR. Additionally, I have extensive experience investigating the internal motions of peptides and the conformational propensity of single amino acid residues using solution NMR. At CNR-ICCOM-Pisa, my group operates a fully equipped laboratory dedicated to fast field cycling NMR. I also have access to a state-of-the-art solid-state NMR facility at the Chemistry Department of Pisa University. Furthermore, I have experience in utilizing the EPR facility at the...
The skills of the IC- Catania complements the structural biology techniques provided by the network, as they are aimed at characterizing the stability and function of newly obtained biological structures.
Academic collaboration, Facility, Patent, Skill
Stability. Combining molecular structures with thermodynamic, spectroscopic, and functional data is essential for establishing a validated structure-activity relationship (SAR) of biological structures. Various experimental techniques are available to evaluate the molecular structures of biological macromolecules. The IC Catania team has advanced laboratories with tools including two nano-calorimeters, Isothermal Titration Calorimetry (ITC), Differential Scanning Calorimetry/Pressure Perturbation Calorimetry (DSC/PPC), Biolayer Interferometry (BLI), as well as spectroscopy instruments like a Spectrophotometer and a CD Spectropolarimeter. DSC uniquely provides thermodynamic parameters (enthalpy, entropy, Gibbs energy) by measuring heat changes during macromolecule folding/unfolding, thus offering insight into molecular stability. ITC measures heat during ligand-target binding...
Vibrational spectroscopy FTIR/Raman/Raman SERS for protein analysis
Academic collaboration, Facility, Research field/topic, Skill, Software
Vibrational spectroscopy FTIR/Raman/Raman SERS for biomolecule analysis ICCOM Pisa has instrumentation and consolidated skills in the field of biomacromolecule identification in biological complex systems (tissues and cells) by FTIR, Raman and Raman-SERS (Bramanti, Legnaioli, Campanella), and conformational analysis of proteins (Bramanti), including skills for extracting information from spectroscopic data using advanced chemometric tools (Campanella). ICCOM team has also a facilities and skills for the characterization of proteins by UV spectroscopy, Fluorescence spectroscopy, and liquid chromatography and for study of surface tension and viscosity of proteins by a home made dynamic surface tension detector (DSTD). These facilities allow strict collaborations with...