Institution: IOM
Departement: DSFTM
Typology: Academic collaboration, Facility, Industrial collaboration, Skill
Location: Q2 Building - Area Science Park Strada Statale 14 km 163,5 - 34149 Basovizza - Trieste
Contacts:
- Luigi De Colibus (decolibus@iom.cnr.it)
- Simone Dal Zilio (dalzilio@iom.cnr.it)
Description:
Cryo-Electron Microscopy (Cryo-EM) is an advanced imaging technique used to determine the three-dimensional (3D) structure of biological molecules and complexes at near-atomic resolution. In this method, the sample is rapidly frozen at temperatures below -150 °C, encapsulating it in vitreous ice. The specimen is then imaged from various angles using an electron microscope, where a beam of electrons passes through the sample, producing a series of two-dimensional (2D) projections. Advanced computational algorithms are employed to reconstruct a 3D model of the sample from these projections. The CMF is equipped with the necessary instruments to conduct cryo-electron microscopy studies, including single-particle analysis and cryo-electron tomography (cryo-ET). The facility has a 200 keV Glacios2 microscope and an Aquilos2 for sample preparation, specifically for cryo-ET.
Team:
-
Martina Conti (conti@iom.cnr.it)
200KeV Glacios2 microscope. Aquilos2 cryo FIB.